This DATSETNAMEreadme.txt file was generated on 2017/08/04 by D.P. Mulvihill

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GENERAL INFORMATION
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1. Title of Dataset
Novel segmentation strategies for fully-automated analysis of yeast images.


2. Author Information

  Principal Investigator Contact Information
        Name:
           Institution: University of Kent
           Address: 
           Email:

  Associate or Co-investigator Contact Information
        Name: Daniel P. Mulvihill
           Institution:University of Kent
           Address:
           Email: d.p.mulvihill@kent.ac.uk

  Associate or Co-investigator Contact Information
        Name:
           Institution:University of Kent
           Address:
           Email:

  Alternate Contact Information
           Name:
           Institution:
           Address:
           Email:

3. Date of data collection (single date, range, approximate date): 2014-1017

4. Geographic location of data collection (where was data collected?): University of Kent

5. Information about funding sources that supported the collection of the data:Royal Society Industry Fellowship, University of Kent GTA.

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DATA & FILE OVERVIEW
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1. File List
   A. Filename:O’Brien_dataset       
      Short description:Zip file of TIF Images and Ground Truth files (Matlab format)        
      
2. Relationship between files: Images of fission yeast cells acquired using different optical pathways       

3. Additional related data collected that was not included in the current data package:

4. Are there multiple versions of the dataset? no
  

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METHODOLOGICAL INFORMATION
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1. Description of methods used for collection/generation of data: 
Samples were visualised using an Olympus IX71 microscope with either PlanApo 100x OTIRFM-SP 1.45 NA PlanApo 60x 1.4 NA oil objectivelens mounted on a PIFOC z-axis focus drive (Physik Instrumente, Karlsruhe, Germany), and illuminated using LED light sources (Cairn Research Ltd, Faversham, UK) with appropriate filters (Chroma, Bellows Falls, VT). An Optosplit device (Cairn Research Ltd) was used to allow simultaneous acquisition of signals from two fluorophores that emitted light of different wavelengths. Samples were visualised using either a QuantEM (Photometrics) EMCCD or Zyla 5.5 (Andor) CMOS camera, and the system was controlled with Metamorph software (Molecular Devices). Each 3D-maximum projection of volume data was calculated from 21 z-plane images, each 0.2 µm apart, and analysed using Metamorph and Autoquant X software. During live-cell imaging, cells were cultured in Edinburgh minimal media using 20 mM L-Glutamic acid as a nitrogen source (EMMG). Cells were grown exponentially at 25ºC for 48hr before being mounted (without centrifugation) onto lectin (Sigma L2380; 1 mg/ml) coated coverslips with an a Bioptechs FCS2 (Bioptechs, Butler, PA), fitted onto an ASI motorised stage (ASI, Eugene, OR) on the above system, with the sample holder, objective lens and environmental chamber held at the required temperature. 

2. Methods for processing the data: <describe how the submitted data were generated from the raw or collected data>

3. Instrument- or software-specific information needed to interpret the data:

4. Standards and calibration information, if appropriate:

5. Environmental/experimental conditions:

6. Describe any quality-assurance procedures performed on the data:

7. People involved with sample collection, processing, analysis and/or submission:

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DATA-SPECIFIC INFORMATION FOR: [FILENAME]
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<create sections for each dataset (or file if appropriate) included>

1. Number of variables:

2. Number of cases/rows: 

3. Variable List
    A. Name: [variable name]
       Description: [description of the variable]
                    Value labels if appropriate

    B. Name: [variable name]
       Description: [description of the variable]
                    Value labels if appropriate

4. Missing data codes:
        Code/symbol        Definition
        Code/symbol        Definition

5. Specialized formats of other abbreviations used