This CaM_acetylation_datasetreadme.txt file was generated on 20220125 by Dan Mulvihill
 
 
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GENERAL INFORMATION
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1. Title of Dataset 
Acetylation stabilises Calmodulin regulated calcium signalling. 
 
2. Author Information

  Principal Investigator Contact Information
        Name: Daniel P Mulvihill	
           Institution: University of Kent
           Address: School of Biosciences, Canterbury, Kent, CT2 7NJ, UK
           Email: d.p.mulvihill@kent.ac.uk
 
  Associate or Co-investigator Contact Information
        Name: Karen Baker
           Institution: University of Kent
           Address: School of Biosciences, Canterbury, Kent, CT2 7NJ, UK
           Email: K.B.Baker@kent.ac.uk
 
  Associate or Co-investigator Contact Information
           Name: Michael A. Geeves
           Institution: University of Kent
           Address: School of Biosciences, Canterbury, Kent, CT2 7NJ, UK
           Email: m.a.geeves@kent.ac.uk
 

3. Date of data collection (single date, range, approximate date):
2012 to 2021 
 
4. Geographic location of data collection (where was data collected?): 
 School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK
 
5. Information about funding sources that supported the collection of the data:
 BBSRC -  BB/J012793/1 & BB/S005544/1
 
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DATA &amp; FILE OVERVIEW
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1. File List
   A. Filename: cam1-gfp naa15D images.zip       
      Short description: Compressed folder containing original image acquisition files of mixtures of cam1-gfp myo52-tdTomato and cam1-gfp naa15∆ cells. Images were acquired using Metamorph software. They can be opened using freely available open source software (e.g. FIJI image J)      

   B. Filename: gfp-cam1 naa15D images.zip       
      Short description: Compressed folder containing original image acquisition files of gfp-cam1 and gfp-cam1 naa15∆ cells. Images were acquired using Metamorph software. They can be opened using freely available open source software (e.g. FIJI image J) 
        
   C. Filename: Biochemistry data files.zip       
      Short description: Compressed folder of individual MS Excel files, each containing raw data for discrete experiment types
 
 
2. Relationship between files: Each zip file contains a discrete set of data.     
 
 
3. Additional related data collected that was not included in the current data package:

None 
 
 
4. Are there multiple versions of the dataset? no

   If yes, list versions:
           Name of file that was updated:
                     i. Why was the file updated? 
                ii. When was the file updated?
           Name of file that was updated:
                      i. Why was the file updated?
                    ii. When was the file updated?
 
 
 
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METHODOLOGICAL INFORMATION
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1. Description of methods used for collection/generation of data: 

All methods and reagents are described in the associated peer reviewed research article (KAR record is: https://kar.kent.ac.uk/92803/)
 
 
2. Methods for processing the data:
 Described in the associated peer reviewed research article (KAR record is: https://kar.kent.ac.uk/92803/)
 
3. Instrument- or software-specific information needed to interpret the data:

Described in the associated peer reviewed research article (KAR record is: https://kar.kent.ac.uk/92803/)
 
4. Standards and calibration information, if appropriate:
 N/A
 
5. Environmental/experimental conditions:
 N/A
 
6. Describe any quality-assurance procedures performed on the data:
 Described in the associated peer reviewed research article (KAR record is: https://kar.kent.ac.uk/92803/)
 
7. People involved with sample collection, processing, analysis and/or submission:
 
K. Baker: Sample collection, processing, and analysis.
M. Geeves: Analysis and submission
D. P. Mulvihill: Analysis and submission 
 
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DATA-SPECIFIC INFORMATION FOR: [cam1-gfp naa15D images.zip] and [gfp-cam1 naa15D images.zip]
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1. Number of variables: 5
 
 
2. Number of cases/rows: NA
 
(A) Name: 		[FP labelled protein]
       Description: 	[Description of the variable]
       Value labels: 	cam1-GFP, gfp-cam1, or myo52tom

(B) Name: [Genetic background]
       Description: [denotes gene deletion strain examined]
       Value labels: 	naa15 or naa15∆

(C) Name: [file number]
       Description: [Description of Fluorescence protein or light path]
       Value labels: GFP, tdTomato or Phase

(D) Name: [Emission Wavelength Captured]
       Description: [Description of Fluorescence protein or light path]
       Value labels: GFP, tdTomato or Phase

(E) Name: [Time point]
       Description: [Image number in time series]
       Value labels: 1,2,3,…

 
4. Missing data codes: none
 

5. Specialized formats of other abbreviations used: none