This Sp-polarity readme.txt file was generated on [2018-06-14] by [Dan Mulvihill]


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GENERAL INFORMATION
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1. Title of Dataset 
Polar recruitment interdependencies between components of the fission yeast polarity network.

Data associated with publication: M. Johnson and D. P. Mulvihill (2018) “Dependency Relationships within the Fission Yeast Polarity Network.” FEBS Letters. 592: 2543-49. doi: 10.1002/1873-3468.13180.”

2. Author Information


  Principal Investigator Contact Information
        Name: Dr. Daniel P. Mulvihill
           Institution:University of Kent
           Address:School of Biosciences, Giles Lane, Canterbury, Kent, CT27NJ, UK
           Email: d.p.mulvihill@kent.ac.uk


  Associate or Co-investigator Contact Information
        Name: Dr. Matthew Johnson
           Institution: University of Kent
           Address:School of Biosciences, Giles Lane, Canterbury, Kent, CT27NJ, UK
           Email:




3. Date of data collection:
2010 to 2014


4. Geographic location of data collection (where was data collected?):
School of Biosciences, University of Kent, Canterbury.


5. Information about funding sources that supported the collection of the data:
This work was supported by funding from the Biotechnology and Biological Sciences Research Council (BB/H016066/1), a CASE industrial bursary from Cairn Research Ltd. and a Royal Society Industry Fellowship.


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DATA & FILE OVERVIEW
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1. File List
   A. Filename:        
      Short description: Compressed ZIPs file of TIF Image datasets for each GFP labelled protein examined in this study.       


2. Relationship between files:
Each ZIP files contain Dataset of Images of fission yeast cells expressing a specific Green Fluorescent Protein (GFP) labelled polarity markers within different deletion background strains mixed onto coverslips with wild type controls (contain red labelled spindle pole bodies).


3. Additional related data collected that was not included in the current data package:
No

4. Are there multiple versions of the dataset?
No   



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METHODOLOGICAL INFORMATION
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1. Description of methods used for collection/generation of data: 

Samples were visualized using an Olympus IX71 microscope with PlanApo 100x OTIRFM-SP 1.45 NA lens mounted on a PIFOC z-axis focus drive (Physik Instrumente, Karlsruhe, Germany), and illuminated using LED light sources (Cairn Research Ltd, Faversham, UK) with appropriate filters (Chroma, Bellows Falls, VT). Samples were visualized using  a QuantEM (Photometrics) EMCCD camera, and the system was controlled with Metamorph software (Molecular Devices). 3D dataset files for fluorescent images are composed of 31 x z-plane images were each 0.2 µm apart. During live-cell imaging, cells were mounted onto coverslips with lectin (Sigma L2380; 1 mg/ml) in a Bioptechs FCS2 (Bioptechs, Butler, PA), fitted onto an ASI motorized stage (ASI, Eugene, OR) on the above system, with the sample holder, objective lens and environmental chamber held at the required temperature. All live-cell imaging was undertaken using EMM2 media supplemented with appropriate amino acids. 


2. Methods for processing the data:
File contains unprocessed raw data files.


3. Instrument- or software-specific information needed to interpret the data:
Images acquire using MDA Metamorph software. Can be visualised using variety of standard image analysis packages. Freely available open source software packages available, such as Fiji (https://imagej.net/ImageJ) or Micromanager (https://micro-manager.org/).

4. Standards and calibration information, if appropriate:
Pixels are 0.16µm x 0.16µm.

5. Environmental/experimental conditions:
Cells were mounted directly from the culture (without centrifugation) onto lectin-coated coverslips and into a Bioptechs FCS2 chamber (Bioptechs, Butler, PA). Cells were imaged at 20 ºC.

6. Describe any quality-assurance procedures performed on the data:
-

7. People involved with sample collection, processing, analysis and/or submission:

Matthew Johnson: Strain generation, cell culture, sample preparation, and image acquisition.
Daniel Mulvihill: Manuscript preparation and data submission.




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DATA-SPECIFIC INFORMATION FOR: [Sp-polarity]
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1. Number of variables: 3


2. Number of cases/rows: N/A


3. Variable List

    (A) Name: 		[GFP labelled protein]
       Description: 	[Description of the variable]
       Value labels: 	bud6-GFP, for3-GFP, mod5-GFP, myo52-GFP, tea1-gfp, tea3-gfp,
			tip1-gfp, tea4-gfp or mal3-gfp


    (B) Name: [Genetic background]
       Description: [denotes gene deletion strain examined]
       Value labels: 	bud6∆, for3∆, mod5∆, myo52∆, tea1∆, tea3∆, tip1∆, tea4∆,
			gef1∆ or mal3∆

    (C) Name: [Emission Wavelength Captured]
       Description: [Description of Fluorescence protein or light path]
       Value labels: GFP, tdTomato or Phase

Example filename with variables in brackets:

tea1gfp(A)sid4tom MIX tea3D(B)tea1gfp(A) 026_w15. tdTomato(C) LED3.TIF


4. Missing data codes:
        Code/symbol        Definition
        Code/symbol        Definition


5. Specialised formats of other abbreviations used