Data associated with the paper Antczak et al. "Environmental conditions shape the nature of a minimal bacterial genome" published in Nature Communications 2019

Antczak, Magdalena and Michaelis, Martin and Wass, Mark N (2019) Data associated with the paper Antczak et al. "Environmental conditions shape the nature of a minimal bacterial genome" published in Nature Communications 2019. [Data Collection]

Description

Of the 473 genes in the genome of the bacterium with the smallest genome generated to date, 149 genes have unknown function, emphasising a universal problem; less than 1% of proteins have experimentally determined annotations. Here, we combine the results from state-of-the-art in silico methods for functional annotation and assigned functions to 66 of the 149 proteins. Proteins that are still not be annotated lack orthologs, lack protein domains, and/ or are membrane proteins. Twenty-four likely transporter proteins are identified indicating the importance of nutrient uptake into and waste disposal out of the minimal bacterial cell in a nutrient-rich environment after removal of metabolic enzymes. Hence, the environment shapes the nature of a minimal genome. Our findings also show that the combination of multiple different state-of-the-art in silico methods for annotating proteins is able to predict functions, even for difficult to characterise proteins and identify crucial gaps for further development.

DOI: 10.22024/unikent/01.01.78
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Magdalena Antczak
Collection period:
From
To
2017
2019
Last Modified: 01 Apr 2021 08:12
Publication Date: 4 June 2019
URI: https://data.kent.ac.uk/id/eprint/78

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